UV-Visible Spectrophotometer

1. Name of the Instrument: UV-Visible Spectrophotometer

2. Working Principle : The UV–Visible Spectrophotometer operates on the Beer-Lambert Law, which states that the absorbance of light by a substance is directly proportional to its concentration. The instrument passes ultraviolet and visible light through a sample solution after selecting a specific wavelength using a monochromator. As the light travels through the sample contained in a cuvette, a portion of the light is absorbed by the analyte while the remaining light is transmitted. The detector measures the amount of absorbed light (absorbance), which is then used to accurately determine the concentration of the substance present in the sample.

3. Applications :
• Quantitative analysis of proteins, DNA, RNA and other biomolecules

3. Types of Studies: :
Molecular Biology, Phytochemistry, Food Technology